Roskams Lab

Protocol:	Link:
Animals
Bulbectomy	Bulbectomy
NMDA microinjection	NMDA microinjection
Triton Ablation	Triton Ablation
Perfusion	Perfusion
Fresh frozen and fixed embedding	Embedding

Immuno
VIP/ABC staining	VIP/ABC staining
Immunofluorescence	IF
Nuclear Immunohistochemistry	Nuclear Immuno

RNA Work
RNA purification	RNA purification
RT-PCR	RT-PCR
In situ hybridization	In situ hybridization

DNA Work
Plasmid mini/maxi preps	Boiling plasmid mini prep Endo-free plasmid maxi prep
DNA extraction	DNA extraction
Genomic DNA preps	Genomic DNA preps
Genotyping	Caspase 3 genotyping Caspase 9 genotyping
DNA gels	DNA gels

Protein Work
Protein lysates (tissue/cells)	Total protein extraction
Protein preps – subcellular
Western blotting	Prep for Immunoblotting Silverstain protocol
Immunoprecipitation	Prep for Immunoprecipitation

Apoptosis Work
TUNEL	Alkaline phosphatase TACS Blue Fluorescence
SsDNA immunodetection	SsDNA
MTT/WST-1	MTT

Tissue Culture
Making media – recipes and how-to	Perfect media every time! Hormone media mix MHM ingredient list Generating LP-OCM media
OP cell line expansion, freezing and reconstit	OP cell line care
OEC prep	Lamina Propria OEC prep
Neurospheres	Neurosphere cultures
FACS selection	FACS protocol
Magnetic Activated Cell sorting	MACS
DRG explants	Embryonic DRG dissection
OEC-CM production	LP-OEC conditioned media