| Protocol: |
Link: |
| Animals |
|
Bulbectomy
|
Bulbectomy |
| NMDA microinjection |
NMDA microinjection |
| Triton Ablation |
Triton
Ablation |
| Perfusion |
Perfusion |
| Fresh frozen and fixed embedding |
Embedding |
| |
|
| Immuno |
|
| VIP/ABC staining |
VIP/ABC staining |
Immunofluorescence
|
IF
|
| Nuclear Immunohistochemistry |
Nuclear
Immuno |
| |
|
| RNA Work |
|
RNA purification
|
RNA purification |
| RT-PCR |
RT-PCR |
| In situ hybridization |
In
situ hybridization |
| |
|
| DNA Work |
|
Plasmid mini/maxi preps
|
Boiling
plasmid mini prep
Endo-free
plasmid maxi prep |
| DNA extraction |
DNA
extraction |
| Genomic DNA preps |
Genomic
DNA preps |
| Genotyping |
Caspase
3 genotyping
Caspase 9 genotyping |
| DNA gels |
DNA
gels |
| |
|
| Protein Work |
|
| Protein lysates (tissue/cells) |
Total
protein extraction |
Protein preps – subcellular
|
|
Western blotting |
Prep
for Immunoblotting
Silverstain protocol |
Immunoprecipitation |
Prep
for Immunoprecipitation |
| |
|
| Apoptosis Work |
|
TUNEL
|
Alkaline
phosphatase
TACS Blue
Fluorescence |
| SsDNA immunodetection |
SsDNA |
| MTT/WST-1 |
MTT |
| |
|
| Tissue Culture |
|
Making media – recipes
and how-to
|
Perfect
media every time!
Hormone media mix
MHM ingredient list
Generating LP-OCM media |
| OP cell line expansion, freezing and reconstit |
OP
cell line care |
| OEC prep |
Lamina
Propria OEC prep |
| Neurospheres |
Neurosphere
cultures |
| FACS selection |
FACS
protocol |
| Magnetic Activated Cell sorting |
MACS |
| DRG explants |
Embryonic
DRG dissection |
| OEC-CM production |
LP-OEC
conditioned media |
| |
|
 |
