NAPS, the UBC service we use for Sanger sequencing, charges 2 CAD for purifying a sequencing reaction; especially if submitting several reactions, there is therefore a reasonably big incentive in doing the purification ourselves. Continue reading
Tag Archives: SPRI beads
How to do effective SPRI beads cleaning
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SPRI beads cleaning is one of the most repetitively used step during library preparations and probably the step where most of us lose a lot of precious DNAs.
Losing DNA scared me so much (because it can be observable) that I hesitated a lot before trying to use beads to concentrate genomic DNA, because usual rate of recovery are ~50%.
Hopefully with some practice and a lot of patience, it is possible to reach 90% recovery. How to lose as little DNA as possible? Here are some guidelines: Continue reading