Cutting a slice from an agarose gel in order to size select DNA or to isolate a PCR product etc. is standard practice in lab genetics. This post is about how I do it in our lab, (as of Feb 2012).

There are three features of my current method that may be unfamiliar to you even if you’ve done this sort of thing before:

1. The Dark Reader transilluminator.
2. SYBR-Gold.
3. Sigma X-Tracta gel punches.

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