Ampicillin Stocks & Usage
* Stock Concentration 50mg/ml in H2O
* Working Concentration 50ug/ml
Preparation of 80ml stock solution
* Ampicillin is kept in the 4C fridge.It is light sensitive.
* Weigh 4g of ampicillin into a small weigh boat.
* Add 80ml of milliQ to a 250ml bottle.
* Add the ampicillin to the milliQ
* Mix/vortex so all the ampicillin goes into solution.
* Filter sterilize the solution into a falcon tube using a 20ml syringe and a 200nm filter.
* Aliquot into 1.5ml eppendorfs.
* Store at -20C.