Oligonucleotide Properties Calculator
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Enter Oligonucleotide Sequence Below
OD and Molecular Weight calculations are for single-stranded DNA or RNA
Nucleotide base codes
Reverse Complement Strand(5' to 3') is:
Number of Fluorescent tags per strand:
6-FAM
TET
HEX
TAMRA
ssDNA
ssRNA
dsDNA
dsRNA
Minimum base pairs required for single primer self-dimerization:
1
2
3
4
5
6
7
8
Minimum base pairs required for a hairpin :
1
2
3
4
5
6
7
8
Physical Constants
Melting Temperature (T
M
) Calculations
Length:
bases
GC content:
%
Molecular Weight:
4
1 ml of a sol'n with an Absorbance of
at 260 nm
is
microMolar
5
and contains
micrograms.
1
°C (Basic)
2
°C (Salt Adjusted)
3
°C (Nearest Neighbor)
n
M
Primer
m
M
Salt (Na
+
)
Thermodynamic Constants
Conditions: 1
M
NaCl at 25°C at pH 7.
RlnK
cal/(°K*mol)
deltaH
Kcal/mol
deltaG
Kcal/mol
deltaS
cal/(°K*mol)
Oligo Calculator version 3.07 (last modified by WAKibbe 03/25/2004)